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Image Search Results
Journal: eLife
Article Title: MorphoGraphX: A platform for quantifying morphogenesis in 4D
doi: 10.7554/eLife.05864
Figure Lengend Snippet: ( A and B ) Control for viewing angle. ( A ) A shoot apex imaged from different directions. A first image stack (in red) was acquired before tilting the Z axis (dashed lines) by approximately 30° and acquiring a second stack (in green). Cells were then segmented on both stacks and their areas compared ( B ). Note that the pairwise cell size differences are random, with no obvious trend related to the viewing angle. Average error per cell is less than 2%. Colorbar: relative surface area increase in percent. Panels ( A ) and ( B ) adapted from Figure 5 of . ( C and D ) Comparison between projected areas and actual 3D volumes. ( C ) The epidermal cells of the apex were projected on the surface and segmented. Heatmap shows percent increase in area over 11 hr of growth. ( D ) The same data was segmented in 3D. Heatmap shows the percent increase in volume of cells, same color scale as in ( C ). Note the close correspondence in cell expansion extracted from surface and volumetric segmentations. ( E ) Difference in size between automatically and manually segmented cells on a tomato shoot apex. Cells fused by auto-segmentation are in bright red, split cells are in dark blue. ( F ) Cell sizes (in μm 2 ) from manual (top) and automatic (bottom) segmentation on a fragment of Drosophila wing disc. Scale bars: 40 μm. DOI: http://dx.doi.org/10.7554/eLife.05864.021
Article Snippet:
Techniques: Control, Comparison